MDA said that the three women showed no signs of life after the collision and were pronounced dead on the scene.
The five killed were residents of Safed, and were all Breslov Hasidim, two of whom had families, according to Israeli media.
Authorities believe that the off-duty ambulance may have been tracking a stolen vehicle, which led the car to drive in the wrong direction, and resulted in the crash.
The new LIVALL smart helmets redefine safety for two-wheel riders, with features like fall detection, SOS messaging, and other life-saving innovations now available in Israel.
Out of the nearly 3,000 people, 1,969 were evacuated for further treatment at hospitals, including 286 who fainted, became dehydrated, or felt unwell due to fasting.
According to preliminary reports, the three killed are parents, Jumana and Naji Amer, and their two-month-old infant daughter, residents of Kfar Qasim.
While the number of people killed as a result of road accidents is minor in European countries, in Israel it has increased by considerable percentages, the State Comptroller revealed.
Pedestrians may be twice as likely to be hit by an electric or a hybrid car as those powered by petrol or diesel, wrote Phil Edwards, Siobhan Moore, and Craig Higgins of the London School of Hygiene.
Police commissioner Kobi Shabtai told reporters two other people were injured in the incident.
Police are investigating both incidents.
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