Ronen Bar is the current head of the Shin Bet (Israel Security Agency), the country's primary internal security service.
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Several members of Knesset applauded Bar for making "the right and appropriate decision," while others decried the move, saying he had already been fired.
Bar made the announcement at the memorial event for fallen Shin Bet personnel at the Shin Bet headquarters.
"I greatly appreciate Bar as a person, but he needs to announce his resignation," Ilan Lotan added.
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In a recording, Shin Bet chief Ronen Bar says he has no desire to stay and faces harassment against his family.
Netanyahu and Shin Bet chief Bar each admit partial truths about October 7, exposing deeper issues of trust and leadership.
Yair Lapid, Benny Gantz, and Yair Golan all denounced Netanyahu, as well as senior activists representing families affected by the October 7 massacre.
The prime minister, in his affidavit to the High Court of Justice, claimed Ronen Bar did not alert him to the dangers of Hamas's invasion.
Throughout the session, numerous MKs were expelled from the discussions for outbursts and calling Committee Chair Simcha Rothman a "bully."